ABSTRACT
Objective To investigate the effects of Jagged1 gene silencing on the expression of VEGF,angiopoietin 2,bFGF,MMP 9,and the migration of human pancreatic cancer cells SW1990 and PANC1.Methods Jagged1 siRNA and control siRNA were transfected into human pancreatic cancer cells SW1990 and PANC1 respectively.The expressions of Jaggedl mRNA and protein were detected by real-time PCR and Western blotting.ELISA was used to detect the concentration of VEGF,angiopoietin 2,bFGF,MMP9 in cell supernatants.Transwell was used to detect the migration of SW1990 and PANC1 cells.Results Jagged1 mRNA and protein were highly expressed in human pancreatic cancer cells SW1990 and PANC1.After transfected with 15 nmol/L Jagged1 siRNA for 72 h,the expression of Jagged1 mRNA in SW1990 was inhibited and reduced by (59.62 ±2.75)％ and in PANC1 reduced by (76.96 ±6.16)％ when compared with that in control siRNA group.The Jagged1 protein expression was almost inhibited.The concentration of VEGF in the culture supernatants of SW1990 and PANC1 cells was significantly decreased[( 867.93 ± 58.69 ) pg/ml vs.(1516.24 ±37.58)pg/ml,951.13 ± 120.49)pg/ml vs.(1413.68 ±33.56)pg/ml,P ＜0.05],but the protein concentration of angiopoietin 2,bFGF,MMP9 were not significantly changed.In addition,after transfected with Jagged1 siRNA,tne expression of VEGF mRNA was (52.26 ± 4.85 )％ of the control levels in SW1990 ( P ＜ 0.05),and (59.75 ± 4.91 ) ％ of the control levels( P ＜ 0.05 ) in PANC1.The number of cell migration of transfected SW1990 and PANC1 was 65.25 ± 5.56 and 57.50 ± 8.58,which were significantly lower than thosein the control siRNA group (122.25±11.09,112.00±12.52,P＜0.05).Conclusions Jagged1 is highly expressed in human pancreatic cancer cells.Jagged1 gene silencing significantly inhibits the production and secretion of VEGF in SW1990 and PANC1 cells,and reduces the migration of cancer cells in vitro.
ABSTRACT
Objective To investigate the changes of Notch signaling pathway activity in human pancreatic cancer cell lines (SW1990, BxPC3 )after gemcitabine induction, and to study its relationship with pancreatic cancer resistant to gemcitabine chemotherapy. Methods The pancreatic cancer cell lines SW1990 and BxPC3 were cultured with different concentrations of gemcitabine for 48 hours. The Notch signaling pathway receptors ( Notch1, Notch2, Notch3, Notch4), ligands (Jagged1, Jagged2) and downstream target Hesl mRNAs expression were detected by quantitative real-time PCR (Q-PCR). Protein levels of Hes1 were determined by Western blotting. Results After treatment with 2 μmol/L gemcitabine for 48 hours, the expression of Notch1, Notch2, Notch3, Jagged1, Jagged2 and Hes1 mRNAs in SW1990 cells were 8.26 ±0.48, 39.12 ±4.87, 0.84 ±0.06, 105.8 ± 17.92, 6.59 ±0.32 and 17.30 ±2.96, which were significantly elevated when compared with those without gemcitabine treatment ( 1.02 ± 0. 15, 15.25 ± 1.28, 0. 12 ± 0.02,32.66 ± 1.98, 1.88 ± 0.29 and 5.02 ± 0.64, P < 0.05 or P < 0. 01 ); the expression in BxPC3 cells was 7.87 ±0.59, 109.4 ± 10.98, 0.74 ±0.19, 62.73 ± 13.50, 2.09 ±0.16 and 15.38 ± 1.06, which were significantly elevated when compared with those without gemcitabine treatment ( 1.14 ±0.43, 58.96 ±2.63,0.10 ± 0.02, 16.95 ± 3.79, 0.98 ± 0.02 and 2.04 ± 0.16, P < 0.05 or P < 0.01 ). The expressions of Hes1protein in SW1990 cells after 1, 2 μmol/L gemcitabine treatment for 48 h were 0.30 ±0.03, 0.42 ±0.03;and the expressions in BxPC3 cells were 0.33 ± 0.02, 0.45 ± 0.03, which were significantly increased when compared with those without gemcitabine treatment (0.13 ± 0.01, F = 33.71,0.09 ± 0.02, F = 38.54, P <0.01 ). Conclusions The Notch signaling pathway is significantly activated in pancreatic cancer cells SW1990 and BxPC3 by gemcitabine, which may be one of the mechanisms of chemoresistance.